Journal: Neuroendocrinology

Article Title: Androgen Excess Increases Food Intake in a Rat Polycystic Ovary Syndrome Model by Downregulating Hypothalamus Insulin and Leptin Signaling Pathways Preceding Weight Gain

doi: 10.1159/000521236

Figure Lengend Snippet: Effects of DHT on insulin and leptin signaling in the hypothalamus of 6-week-old, prepubertal, nonobese rats. a, e Representative immunoblots showing phosphorylated and total IR, AKT, GSK, JAK2, and Stat3 levels, with β-actin as the loading control. b–d, f, g Densitometry analysis and comparison of phosphorylated forms versus total amounts of different proteins. DHT treatment significantly decreased the phosphorylation levels of these signaling proteins. h Expression levels of feeding-related genes in the hypothalamus of 6-week-old DHT and control rats. Note the significant increase in the mRNA levels of NPY and Agrp genes. Experiments were performed in 3 rats for each group. Data are expressed in mean ± SEM ( n = 6). * p < 0.05, ** p < 0.01. SEM, standard error.

Article Snippet: β-Actin , Abcam , ab8226 , 1:10,000.

Techniques: Western Blot, Expressing

Journal: Neuroendocrinology

Article Title: Androgen Excess Increases Food Intake in a Rat Polycystic Ovary Syndrome Model by Downregulating Hypothalamus Insulin and Leptin Signaling Pathways Preceding Weight Gain

doi: 10.1159/000521236

Figure Lengend Snippet: Effect of DHT on insulin and leptin signaling in the hypothalamus of 7-week-old pair-fed rats. a, f Representative immunoblots showing phosphorylated and total IR, AKT, GSK, JAK2, and Stat3 levels, with β-actin as the loading control. b–e, g–j Densitometry analysis and comparison of phosphorylated versus total amounts of proteins. DHT treatment significantly decreased the phosphorylation levels of these signaling factors after pair feeding. Experiments were performed in 3 rats for each group. Data are expressed in mean ± SEM. * p < 0.05, ** p < 0.01. SEM, standard error.

Article Snippet: β-Actin , Abcam , ab8226 , 1:10,000.

Techniques: Western Blot

Journal: Neuroendocrinology

Article Title: Androgen Excess Increases Food Intake in a Rat Polycystic Ovary Syndrome Model by Downregulating Hypothalamus Insulin and Leptin Signaling Pathways Preceding Weight Gain

doi: 10.1159/000521236

Figure Lengend Snippet: Effect of central leptin on the hypothalamus. a Leptin levels in the CSF were significantly decreased in DHT rats than in control rats. b ICV injection of leptin inhibited the improvement of food intake caused by DHT. c Representative immunoblots showing phosphorylated and total Stat3 levels, with β-actin as the loading control. d Densitometry analysis and comparison of phosphorylated forms versus total amounts of proteins. The phosphorylation of STAT3 was also increased in the DHT rats. Data were expressed as mean ± SEM. * p < 0.05. SEM, standard error.

Article Snippet: β-Actin , Abcam , ab8226 , 1:10,000.

Techniques: Injection, Western Blot

Journal: iScience

Article Title: CD38 regulates ovarian function and fecundity via NAD + metabolism

doi: 10.1016/j.isci.2023.107949

Figure Lengend Snippet:

Article Snippet: Mouse beta actin monoclonal antibody (BA3R), HRP , Fisher Scientific , , RRID: AB_MA515739HRP.

Techniques: Recombinant, Plasmid Preparation, Modification, Electron Microscopy, Saline, Blocking Assay, Staining, Marker, Sterility, Membrane, Western Blot, Stripping, Lysis, Avidin-Biotin Assay, Bicinchoninic Acid Protein Assay, RNAscope, Positive Control, Negative Control, Software, Fluorescence, Microscopy, Imaging, Flow Cytometry, Mass Spectrometry